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ifnar1 monoclonal antibody functional grade  (Fisher Scientific)
90
Fisher Scientific ifnar1 monoclonal antibody functional grade
Dexamethasone broadly inhibits IFNβ-dependent gene expression in LPS-treated macrophages. (A) Mouse BMDMs (three independent isolates per condition) were treated with vehicle, 10 ng/ml LPS for 1 h or 4 h, or 10 ng/ml LPS + 100 nM Dex for 4 h. mRNA was harvested, quantified by microarray and analyzed using Partek. Transcripts robustly induced by LPS at 4 h (fold increase > 5, pAdj < 0.05) were identified and their responses to addition of Dex illustrated by volcano plot. Black dots represent LPS-induced transcripts whose expression was significantly altered by addition of Dex (fold change > 2, pAdj < 0.05). The transcripts highlighted in green are discussed in the text. (B) LPS-induced and Dex-suppressed transcripts (black dots in left quadrant of (A) were subjected to GO analysis using default parameters in Panther. Significantly enriched GO terms were filtered manually and using REVIGO to eliminate redundant terms. The top 14 remaining terms were plotted according to enrichment, FDR, gene number and gene ratio. (C) Gene set enrichment analysis was performed using the comparison of LPS vs. LPS + Dex as a quantitative data set, and the top 70 <t>IFNAR1-dependent</t> transcripts from as a categorical gene list. Dex-inhibited transcripts are clustered at the left, and Dex-enhanced transcripts at the right. ES, enrichment score; NES, normalized enrichment score; q, false discovery rate. (D) Gene set enrichment analysis was performed as in C, except using the 93 most robustly IFNβ-inducible genes from . (E) For 96 genes that were robustly induced by LPS in our data set and interferon-regulated according to or gene expression was normalized to the 4 h value, and mean expression was plotted in Tukey box-and-whisker format. Outliers exceed the median by more than 1.5x interquartile range. ****, p < 0.001.
Ifnar1 Monoclonal Antibody Functional Grade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ifnar1 monoclonal antibody functional grade - by Bioz Stars, 2026-05
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ifnar1 antibody center blocking peptide  (Abcepta)
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Dexamethasone broadly inhibits IFNβ-dependent gene expression in LPS-treated macrophages. (A) Mouse BMDMs (three independent isolates per condition) were treated with vehicle, 10 ng/ml LPS for 1 h or 4 h, or 10 ng/ml LPS + 100 nM Dex for 4 h. mRNA was harvested, quantified by microarray and analyzed using Partek. Transcripts robustly induced by LPS at 4 h (fold increase > 5, pAdj < 0.05) were identified and their responses to addition of Dex illustrated by volcano plot. Black dots represent LPS-induced transcripts whose expression was significantly altered by addition of Dex (fold change > 2, pAdj < 0.05). The transcripts highlighted in green are discussed in the text. (B) LPS-induced and Dex-suppressed transcripts (black dots in left quadrant of (A) were subjected to GO analysis using default parameters in Panther. Significantly enriched GO terms were filtered manually and using REVIGO to eliminate redundant terms. The top 14 remaining terms were plotted according to enrichment, FDR, gene number and gene ratio. (C) Gene set enrichment analysis was performed using the comparison of LPS vs. LPS + Dex as a quantitative data set, and the top 70 IFNAR1-dependent transcripts from as a categorical gene list. Dex-inhibited transcripts are clustered at the left, and Dex-enhanced transcripts at the right. ES, enrichment score; NES, normalized enrichment score; q, false discovery rate. (D) Gene set enrichment analysis was performed as in C, except using the 93 most robustly IFNβ-inducible genes from . (E) For 96 genes that were robustly induced by LPS in our data set and interferon-regulated according to or gene expression was normalized to the 4 h value, and mean expression was plotted in Tukey box-and-whisker format. Outliers exceed the median by more than 1.5x interquartile range. ****, p < 0.001.

Journal: Frontiers in Immunology

Article Title: Dexamethasone impairs the expression of antimicrobial mediators in lipopolysaccharide-activated primary macrophages by inhibiting both expression and function of interferon β

doi: 10.3389/fimmu.2023.1190261

Figure Lengend Snippet: Dexamethasone broadly inhibits IFNβ-dependent gene expression in LPS-treated macrophages. (A) Mouse BMDMs (three independent isolates per condition) were treated with vehicle, 10 ng/ml LPS for 1 h or 4 h, or 10 ng/ml LPS + 100 nM Dex for 4 h. mRNA was harvested, quantified by microarray and analyzed using Partek. Transcripts robustly induced by LPS at 4 h (fold increase > 5, pAdj < 0.05) were identified and their responses to addition of Dex illustrated by volcano plot. Black dots represent LPS-induced transcripts whose expression was significantly altered by addition of Dex (fold change > 2, pAdj < 0.05). The transcripts highlighted in green are discussed in the text. (B) LPS-induced and Dex-suppressed transcripts (black dots in left quadrant of (A) were subjected to GO analysis using default parameters in Panther. Significantly enriched GO terms were filtered manually and using REVIGO to eliminate redundant terms. The top 14 remaining terms were plotted according to enrichment, FDR, gene number and gene ratio. (C) Gene set enrichment analysis was performed using the comparison of LPS vs. LPS + Dex as a quantitative data set, and the top 70 IFNAR1-dependent transcripts from as a categorical gene list. Dex-inhibited transcripts are clustered at the left, and Dex-enhanced transcripts at the right. ES, enrichment score; NES, normalized enrichment score; q, false discovery rate. (D) Gene set enrichment analysis was performed as in C, except using the 93 most robustly IFNβ-inducible genes from . (E) For 96 genes that were robustly induced by LPS in our data set and interferon-regulated according to or gene expression was normalized to the 4 h value, and mean expression was plotted in Tukey box-and-whisker format. Outliers exceed the median by more than 1.5x interquartile range. ****, p < 0.001.

Article Snippet: IFNAR1 Monoclonal Antibody Functional Grade , 1:100 (10 ug/mL) , (MAR1-5A3), Cat: 16-5945-85 , Fisher Scientific.

Techniques: Gene Expression, Microarray, Expressing, Comparison, Whisker Assay

Antibodies used in this study.

Journal: Frontiers in Immunology

Article Title: Dexamethasone impairs the expression of antimicrobial mediators in lipopolysaccharide-activated primary macrophages by inhibiting both expression and function of interferon β

doi: 10.3389/fimmu.2023.1190261

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: IFNAR1 Monoclonal Antibody Functional Grade , 1:100 (10 ug/mL) , (MAR1-5A3), Cat: 16-5945-85 , Fisher Scientific.

Techniques: Functional Assay, Control